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Plant and Cell Physiology, 1990, Vol. 31, No. 4 505-511
© 1990

Article

Metabolic Alterations Associated With Abscisic Acid-Induced Frost Hardiness in Bromegrass Suspension Culture Cells1

Karen K. Tanino2, Tony H. H. Chen, Leslie H. Fuchigami and Conrad J. Weiser

Department of Horticulture, Oregon State University Corvallis, OR 97331-2911, U.S.A.

Metabolic alterations associated with the induction of freezing tolerance by abscisic acid (ABA) were characterized by chemical analysis and by [U-l4C]sucrose partitioning into cellular constituents in bromegrass (Bromus inermis Leyss cv. Manchar) cell suspension cultures. ABA caused a significant elevation in dry matter, particularly in the fraction insoluble in 85% ethanol, that was highly correlated with enhanced frost tolerance. Cell walls, the largest component of the insoluble fraction, increased significantly as frost tolerance increased throughout the ABA treatment period. ABA stimulated total [14C]sucrose uptake by cells from 7% on day 1 to 97% on day 7 compared to control cells. Partitioning studies detected a significant increase in 14CO2 evolution at 3, 5 and 7 days after ABA treatment and a significantly higher incorporation of [14C]sucrose into the ethanol insoluble fraction after 5 and 7 days of treatment. Organic acid depletion in ABA-treated cells was also highly correlated with the increase in hardiness. The concentration of total sugars was higher in ABA-treated cells. The results indicate that most of the metabolic changes during ABA-induced acclimation were similar to changes reported for cells acclimated in response to low temperature.

1Oregon Agricultural Experiment Station Technical Paper No. 9052

2Present address: Department of Horticulture, University of Saskatchewan, Saskatoon, Sask. Canada S7N 0W0

(Received November 1, 1989; Accepted March 13, 1990)
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