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Plant and Cell Physiology, 1990, Vol. 31, No. 4 429-432
© 1990

Article

Nitrate Reductase Activity and Nitrite in Native Pyrenoids Purified from the Green Alga Bryopsis maxima

Yasuyuki Okabe and Mitsumasa Okada

Department of Biomolecular Science, Faculty of Science, Toho University 2-2-1 Miyama, Funabashi, Chiba, 274 Japan

The native, starchless pyrenoids purified from Bryopsis maxima showed NADH-nitrate reductase [NR, EC 1.6.6.1 [EC] ] activity and contained nitrite. The specific activity of NR was 0.024 µmol NO2 formed per min per mg of protein. The value was 80 times greater than that in the crude extract of chloroplasts. The amount of nitrite in the pyrenoids was 2.37 µmol per mg of protein, showing that nitrite was concentrated by a factor of 66 times. These results suggest a physiological role for pyrenoids in the assimilation of nitrate.

(Received November 15, 1989; Accepted February 27, 1990)
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