Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Koyama, H.
Right arrow Articles by Yamaya, T.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Koyama, H.
Right arrow Articles by Yamaya, T.
Agricola
Right arrow Articles by Koyama, H.
Right arrow Articles by Yamaya, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1990, Vol. 31, No. 2 173-177
© 1990

Article

Utilization of Anhydrous Aluminum Phosphate as a Sole Source of Phosphorous by a Selected Carrot Cell Line

Hiroyuki Koyama1, Kunihiko Ojima1 and Tomoyuki Yamaya2

1Laboratory of Plant Nutrition, Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, 981 Japan
2Laboratory of Plant Cell Engineering, Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University 1-1 Tsutsumidori-Amamiyamachi, Aoba-Ku, Sendai, Miyagi, 981 Japan

Two variants of carrot (Daucus carota L. cv. MS Yonsun) cell lines, which had been selected with Al-phosphate as a sole source of phosphorous, were characterized on their mechanisms of phosphate-utilization from Al-phosphate. Both cell lines excreted citrate into the medium. The amount of citrate excretion was highly correlated with cell growth in the presence of Al-phosphate. There was about a 1 to 1 correlation between solublized-Al and excreted-citrate in the medium during cell growth. These results suggest that 1) the citrate could chelate with Al, at a 1 to 1 ratio, in Al-phosphate, 2) the citrate-chelated Al remains outside the cells, and 3) solublized phosphate from Al-phosphate is utilized for the growth of carrot cells. The characteristics of the selected cells were very stable, since the rate of citrate-excretion showed no change after subculturing 25 passages without Al-phosphate.

(Received August 7, 1989; Accepted October 19, 1989)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
T. Ohno, H. Koyama, and T. Hara
Characterization of Citrate Transport through the Plasma Membrane in a Carrot Mutant Cell Line with Enhanced Citrate Excretion
Plant Cell Physiol., February 15, 2003; 44(2): 156 - 162.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. Koyama, A. Kawamura, T. Kihara, T. Hara, E. Takita, and D. Shibata
Overexpression of Mitochondrial Citrate Synthase in Arabidopsis thaliana Improved Growth on a Phosphorus-Limited Soil
Plant Cell Physiol., September 1, 2000; 41(9): 1030 - 1037.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.