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Plant and Cell Physiology, 1989, Vol. 30, No. 2 267-275
© 1989

Article

Changes in the Carbonic Anhydrase Activity and the Rate of Photosynthetic O2 Evolution during the Cell Cycle of Chlorella ellipsoidea C-27

Mayumi Nara, Yoshihiro Siraiwa and Toyoyasu Hirokawa1

Department of Biology, Faculty of Science, Niigata University Ikarashi 2-8050, Niigata, 950-21 Japan

1To whom correspondence should be addressed.

Rhythmical changes in carbonic anhydrase activity(CA) and in photosynthesis were observed during the cell cycle of Chlorella ellipsoidea C-27 synchronized at various concentrations of dissolved CO2 (dCO2 with a regime of 16 h of light and 8 h of darkness. At a constant low concentration of dCO2 (11 {diaeresis}M), intracellular CA activity showed obvious fluctuations with a peak at 8 h after the initiation of illumination, while extracellular CA activity, located on the cell surface, showed only minor fluctuations although the activity was as high as the maximum activity of intracellular CA. In contrast, obvious changes in the activities of intra- and extracellular CA activities were not observed at a high concentration of dCO2 (520 {diaeresis}M). The ratio of photosynthetic activity at limiting versus saturating concentrations of dCO2, which is indicative of the affinity of cells for CO2, showed clear rhythmical changes during the cell cycle and the ratio was higher in low-CO2 cells than in high-CO2 cells. The changes in the ratio seemed to reflect the changes in CA activity.

When the cells that had been synchronized under high CO2 conditions were transferred to low CO2 conditions at any given stage in the cell cycle, CA activity was induced in every case but the capacity for induction of CA was greater in young cells than in mature cells. This result suggests that the capacity of cells to induce CA over the course of the cell cycle is closely related to endogenous aging of the cell.

(Received August 29, 1988; Accepted December 28, 1988)
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