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Plant and Cell Physiology, 1962, Vol. 3, No. 1 53-66
© 1962

Article

SYNCHRONIZATION OF BUDDING CYCLE IN YEAST CELLS, AND EFFECT OF CARBON MONOXIDE AND NITROGEN-DEFICIENCY ON THE SYNCHRONY

YOSHIAKI NOSOH and ATUSI TAKAMIYA

Laboratory of Biology, Tokyo Institute of Technology, and Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo

1. A procedure was devised to synchronize the budding cycle of baker's yeast. The method consists of preliminary selection of the cells by differential centrifugation, combined with treatment at 40° for 1 hr. On further incubation of the pre-treated cells in the fresh medium at 27°, a synchronized budding of the whole population occurs. followed by equally well synchronized maturation and separation of the buds formed.

2. The effect of CO on the budding cycle was investigated by introducing the gas at various stages of the cycle. The most marked effect was observed when it was introduced in the induction period of the cycle, resulting in a prolongation of the induction period. Anaerobiosis was found to cause inhibition similar to that caused by CO described above.

3. 2, 4-Dinitrophenol, when added at the beginning of the induction period, at a low concentration (1.8x 10–4M), was found not to abolish the first budding, but to prolong bud-maturation markedly. At higher concentrations of the poison, the first budding was markedly retarded, followed by the suppression of bud-maturation. The budding was completely suppressed at l.8x 10–3M of the poison.

4. The absence of N-source in the incubation medium did not significantly affect the first budding, but completely abolished the capacity of the cells (both mothers and daughters) for the second budding. The lost capacity could be recovered by supplementing the deficient medium with an N-source (urea), but when the timing of the supply was delayed, the recovery became less and less satisfactory. The presence of CO in the gas atmosphere was found to enhance the deleterious effect of N-deficiency.

(Received September 2, 1961; )
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