Plant and Cell Physiology, 1988, Vol. 29, No. 6 1053-1062
© 1988
Article |
Effects of Propyzamide on Tobacco Cell Microtubules In Vivo and In Vitro
1Department of Biology, Faculty of Science, Osaka University Toyonaka, Osaka 560, Japan
2Pesticides Research Laboratory, Takarazuka Research Center, Sumitomo Chemical Co. Ltd. Takarazuka, Hyogo 665, Japan
Treatment with propyzamide at 2 × 10-6 M or at higher concentrations arrested the cell cycleat metaphase in tobacco BY-2 cells. Metaphase cells having disorganized spindle microtubulesand scattered chromosomes began to appear within several minutes of the addition of propyzamide. Within 30 min, disrupted spindle microtubules and dispersed chromosomes were seenin all metaphase cells. Propyzamide at 2 × 10-6 M or at higher concentrations also disrupted cortical microtubules, but disruption of cortical microtubules required more time than disruption of spindle microtubules. The effect of propyzamide on microtubules was found to be readily reversible. The cells arrested at metaphase by 2 × 10-6 M propyzamide resumed mitosis within 2 h from the termination of treatment with propyzamide. Spindle microtubules reappeared within 15 min from the termination of treatment with propyzamide, and the cortical microtubules within 1 h. Tubulin was isolated from tobacco BY-2 cells by column chromatography on ethyl Nphenylcarbamate-Sepharose 4B. On incubation with EGTA, Mg2+ and DMSO, the purified tobacco tubulin polymerized into microtubules. Propyzamide at 1 × 10-4 M completely inhibitedthe polymerization of tobacco tubulin, but did not inhibit polymerization of bovine braintubulin. Tobacco tubulin was adsorbed onto a column of propyzamide-analogue-linked Sepharose 4B and then purified by chromatography on this column.
(Received February 15, 1988; Accepted June 29, 1988)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. M. Castellano and R. Sablowski Phosducin-Like Protein 3 Is Required for Microtubule-Dependent Steps of Cell Division but Not for Meristem Growth in Arabidopsis PLANT CELL, April 1, 2008; 20(4): 969 - 981. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miklis, C. Consonni, R. A. Bhat, V. Lipka, P. Schulze-Lefert, and R. Panstruga Barley MLO Modulates Actin-Dependent and Actin-Independent Antifungal Defense Pathways at the Cell Periphery Plant Physiology, June 1, 2007; 144(2): 1132 - 1143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Goto and T. Asada Excessive Expression of the Plant Kinesin TBK5 Converts Cortical and Perinuclear Microtubules into a Radial Array Emanating From a Single Focus Plant Cell Physiol., May 1, 2007; 48(5): 753 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Laporte, G. Vetter, A.-M. Loudes, D. G. Robinson, S. Hillmer, C. Stussi-Garaud, and C. Ritzenthaler Involvement of the Secretory Pathway and the Cytoskeleton in Intracellular Targeting and Tubule Assembly of Grapevine fanleaf virus Movement Protein in Tobacco BY-2 Cells PLANT CELL, September 1, 2003; 15(9): 2058 - 2075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Inada and T. Shimmen Involvement of Cortical Microtubules in Plastic Extension Regulated by Gibberellin in Lemna minor Root Plant Cell Physiol., April 1, 2001; 42(4): 395 - 403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Young and V. T. Lewandowski Covalent Binding of the Benzamide RH-4032 to Tubulin in Suspension-Cultured Tobacco Cells and Its Application in a Cell-Based Competitive-Binding Assay Plant Physiology, September 1, 2000; 124(1): 115 - 124. [Abstract] [Full Text]
|
|
|
|
 |
|
 J Chang-Jie and S Sonobe Identification and preliminary characterization of a 65 kDa higher-plant microtubule-associated protein J. Cell Sci., January 8, 1993; 105(4): 891 - 901. [Abstract] [PDF]
|
|