Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Gamier, J.
Right arrow Articles by Guyon, D.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Gamier, J.
Right arrow Articles by Guyon, D.
Agricola
Right arrow Articles by Gamier, J.
Right arrow Articles by Guyon, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1987, Vol. 28, No. 6 1117-1131
© 1987

Article

Characterization of New Strains of Photosynthesis Mutants of Chlamydomonas Reinhardtii IV Impaired Excitation Energy Transfer in Three Low Fluorescent Mutants

Jacques Gamier, Jeannine Maroc and Denise Guyon

Laboratoire de Photosynthese (E.R. n° 306), C.N.R.S. F-91190 Gif sur Yvette, France

Three new mutants of Chlamydomonas reinhardtii were characterized, which showed maximum Chl fluorescence yields close to the initial minimum level and clearly lower than the wild type maximum yield. Two of these mutants, mf 1 and mf 2, also showed anomalies characteristic of mutants lacking functional PS II: absence of PS II-related photochemical activity and of the variable component of fluorescence, deficiencies of high potential Cyt b-559, of the Chl-protein complexes CP III and CP IV and of five PS II-related proteins. The third mutant, mf 3, was able to perform photosynthesis and did not show any of the above deficiencies. Low-temperature fluorescence emission spectra indicated clearly that, whatever the oxido-reduction state of the plastoquinone pool, the emission of the main light-harvesting antenna at 682 nm was weaker and the emission of the PS I core antenna at 712–716 nm was more important in mf 1 and mf 2 than in a "classical" PS II-deficient mutant. In mf 3, unlike the wild type, the PS II-related emissions at 686 and 696 nm were relatively less important than that at 712–716 nm. Low-temperature fluorescence excitation spectra showed that, in the three mutants, the light captured by the main Chl a+b antenna, CP II, was the most efficient in inducing PS I fluorescence.

These results indicate that, in the three mutants, the energy captured by the main light-harvesting antenna is always preferentially transferred towards PS I. They will be discussed in terms of membrane defects leading to an impaired regulation mechanism for the distribution of the excitation energy between the two photosystems.

(Received November 4, 1986; Accepted June 22, 1987)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.