Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Nemoto, Y.
Right arrow Articles by Furuya, M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Nemoto, Y.
Right arrow Articles by Furuya, M.
Agricola
Right arrow Articles by Nemoto, Y.
Right arrow Articles by Furuya, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1987, Vol. 28, No. 6 1043-1049
© 1987

Article

Photocontrol of Nuclear DNA Replication in Chattonella antiqua (Raphidophyceae)

Yasuyuki Nemoto1, Tsuneyoshi Kuroiwa2,3 and Masaki Furuya1,4

1Department of Biology, Faculty of Science, University of Tokyo Hongo, Tokyo 113, Japan
2Department of Cell Biology, National Institute for Basic Biology Myodaijicho, Okazaki 444, Japan

The timing of nuclear DNA replication was examined in a synchronized cell population of a red-tide flagellate, Chattonella antiqua, using fluorescence microspectrophotometry with a DNA-specific fluorochrome, 4',6-diamidino-2-phenylindole (DAPI). Under alternating 12-h periods of light and dark (12L12D), nuclear DNA began to increase synchronously ca. 10 h after the onset of light irradiation. Even when the light-off timing of the light period or the whole span of the 12-h light period was shifted after synchronization under 12L12D cycles, the timing of the beginning of nuclear DNA replication was invariably ca. 10 h from the onset of light irradiation. When irradiation was not given, there was no increase of nuclear DNA. The conclusion reached was that light irradiation is necessary for nuclear DNA replication in Chattonella antiqua and that the timing of the replication is dependent upon only the timing of the onset of the last irradiation. In other words, a light-on signal induces the transition of cell nuclei from the G1 into the S phase and also determines the timing of this event. When not irradiated, cells are arrested in the G1 phase.

3 Present address: Department of Biology, Faculty of Science, University of Tokyo, Hongo, Tokyo 113, Japan.

4 Present address: Frontier Research Programs, RIKEN, Wako-city, Saitama 351-01, Japan.

(Received February 28, 1987; Accepted June 5, 1987)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.