Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Shimmen, T.
Right arrow Articles by MacRobbie, E. A. C.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Shimmen, T.
Right arrow Articles by MacRobbie, E. A. C.
Agricola
Right arrow Articles by Shimmen, T.
Right arrow Articles by MacRobbie, E. A. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1987, Vol. 28, No. 6 1023-1031
© 1987

Article

Characterization of Two Proton Transport Systems in the Tonoplast of Plasmalemma-Permeabilized Nitella Cells

Teruo Shimmen1,2 and Enid A. C. MacRobbie

Botany School, University of Cambridge Downing Street, Cambridge CB2 3EA, United Kingdom

2Correspondence and reprints: Department of Botany, Faculty of Science, University of Tokyo, Hongo, Tokyo 113, Japan.

ATP-dependent and PPi-dependent H+-transport systems of the tonoplast were characterized in plasmalemma-permeabilized Nitella cells, where direct access to the protoplasmic surface of the tonoplast was possible. Since H+ transport across the tonoplast can be measured in situ, the identity of the membrane responsible for H+ pumping is unequivocal. H+ transport was evaluated by the accumulation of neutral red. While both transport systems were obligately dependent on Mg2+, the two transport systems showed completely different sensitivity to NO3– and K+, suggesting the presence of two types of H+-pumps in Nitella tonoplast. NO3– applied to the protoplasmic surface, completely and reversibly inhibited ATP-dependent transport but had no effect on PPi-dependent transport. By contrast, NO3– applied into the vacuole by the vacuolar perfusion technique did not inhibit ATP-dependent or PPi-dependent H+ transport. Replacement of K+ with the organic cation, BTP, inhibited PPi-dependent transport but not the ATP-dependent one, indicating that PPi-dependent transport is K+ dependent. The sensitivities of the H+ transport systems found in the tonoplast of Nitella are quite similar to those of higher plant tonoplasts.

1 Present address: Department of Botany, Faculty of Science, University of Tokyo, Hongo, Tokyo 113, Japan.

(Received February 21, 1987; Accepted May 27, 1987)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
T. Nakayasu, K. Kawauchi, H. Hirata, and T. Shimmen
Demonstration of Cl- Requirement for Inhibition of Vacuolar Acidification by Cycloprodigiosin in Situ
Plant Cell Physiol., July 1, 2000; 41(7): 857 - 863.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.