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Plant and Cell Physiology, 1986, Vol. 27, No. 8 1553-1563
© 1986

Article

Effects of Light on the Metabolism of Glycerolipids in Plastidial and Cytoplasmic Membranes of Wheat Leaves: Studies in Vivo and in Vitro

Jacqueline Bahl and Marie-José Alpha

Laboratoire dt Biologie Végétale IV, C.N.R.S. (U.A. 1180), Université Pierre et Marie Curie 12 rue Cuvier, 75005 Paris, France

Wheat leaves were labelled with [l-14C]-glycerol or [l-14C]-acetate and chase experiments performed in the dark or under light. In plastids, both in the dark and under light, the results indicate a transfer of [l-14C]-glycerol from phospholipids to galactolipids and of [l-14C]-acetate from phosphatidylcholine (PC) to monogalactosyl diacylglycerol (MGDG). They also argue for a transfer of [l-14C]-glycerol and [1-14C]-acetate from phosphatidylcholine (PC) to phosphatidylethanolamine (PE) in extraplastidial membranes. During chase experiments in the dark, the chloroplasts accumulated higher amounts of radioactive precursor in saturated fatty acids. In the dark or under light, oleoyl-PC labelling equally decreased in plastids, but decreased much more under light in extraplastidial membranes. Light enhanced polyunsaturated fatty acid synthesis, mainly in MGDG, PC, PE and plastidial phosphatidylglycerol (PG).

In the dark or under light, all glycerolipids were labelled when purified plastids were incubated with [l-14C]-acetate. Light stimulated the incorporation of the label in palmitoyl-MGDG, PG and sulfoquinovosyldiacylglycerol (SL) and also the transfer of oleate from PC to MGDG. Only under light and when extraplastidial membranes were added to isolated plastids, linoleoyl-MGDG, PG and PC were slightly labelled.

These results argue for a stimulating effect of light on glycerolipid synthesis in wheat leaf chloroplasts, on the transfer of oleate from PC to MGDG and on the desaturase activity.

(Received March 8, 1986; Accepted September 26, 1986)
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