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Plant and Cell Physiology, 1986, Vol. 27, No. 8 1533-1539
© 1986

Article

Malate Decarboxylation by Mitochondria of the Inducible Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum

Klaus Winter1, Geoffrey P. Arron2 and Gerald E. Edwards3,4

Department of Horticulture; University of Wisconsin Madison, Wisconsin 53706, U.S.A.

4To whom requests for reprints should be addressed.

Mitochondria isolated from leaves of Mesembryanthemum crystallinum oxidized malate by both NAD malic enzyme and NAD malate dehydrogenase. Rates of malate oxidation were higher in mitochondria from plants grown at 400 mil NaCl in the rooting medium and performing Crassulacean acid metabolism (CAM) than in mitochondria from plants grown at 20 mM NaCl and exhibiting C3-photosynthetic CO2 fixation. The mitochondria isolated from plants both in the CAM and C3 modes were tightly coupled and gave high respiratory control. At optimum pH for malate oxidation (pH 7.0), pyruvate was the major product in mitochondria from CAM-M. crystallinum, whereas mitochondria from C3-M. crystallinum produced predominantly oxaloacetate. Both the extracted NAD malic enzyme in the presence of CoA and the oxidation of malate to pyruvate by the mitochondria from plants in the CAM mode had a pH optimum around 7.0 with activity declining markedly above this pH. The activity of NAD-malic enzyme, expressed on a cytochrome c oxidase activity basis, was much higher in mitochondria from the CAM mode than the C3 mode. The results indicate that mitochondria of this species are adapted to decarboxylate malate at high rates during CAM.

1Current address: Lehrstuhl für Botanik II, Universität Wurzburg, Mittlerer Dallenbergweg 64, 8700 Würzburg, West Germany.

2Current address: KD 120, Chemical Research Division, Ontario Hydro, 800 Kipling Avenue, Toronto, Ontario M8Z5S4, Canada.

3Current address: Department of Botany, Washington State University, Pullman, Washington 99164-4230, U.S.A.

(Received March 13, 1986; Accepted September 18, 1986)
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K. Winter and J. A.M. Holtum
Environment or Development? Lifetime Net CO2 Exchange and Control of the Expression of Crassulacean Acid Metabolism in Mesembryanthemum crystallinum
Plant Physiology, January 1, 2007; 143(1): 98 - 107.
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