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Plant and Cell Physiology, 1986, Vol. 27, No. 8 1469-1474
© 1986


Article

Epidermal Abscisic Acid as Detected by Immunofluorescence. Effect of Proline

N. R. Curvetto, S. E. Delmastro and R. E. Brevedan

Dpto. de Agronomia, Universidad National del Sur, CERZOS-CONICET 8000 Bahia Blanca, Argentina

The effect of proline supplied to detached young leaves of Vicia faba L. var. major on the level of epidermal ABA was investigated. After 1 h treatment with 5 min proline, fluorescent isothiocyanate (FITC) labeling of sonicated epidermal peelings indicated a significant increase in the level of endogenous ABA. In a simultaneous experiment on the same material, the effect of 10–7 M ABA on epidermal FITC. fluorescence labeling was also studied. A marked increase in epidermal FITC fluorescence was observed. These results suggest an involvement of proline in the enhancement of ABA bound to the plasma membrane of epidermal cells.

(Received April 11, 1986; Accepted September 1, 1986)
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