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Plant and Cell Physiology, 1986, Vol. 27, No. 7 1419-1422
© 1986

Short Communication

Cell-Free Synthesis of Rice Prolamin

Hiroshi Yamagata1, Koichi Tamura2, Kunisuke Tanaka1,4 and Zenzaburo Kasai3

1Department of Biochemistry, College of Agricultural Chemistry, Kyoto Prefectural University Shimogamo, Kyoto 606, Japan
2Central Research Division, Fujisawa Pharmaceutical Co. Ltd. Osaka 532, Japan
3Department of Agricultural Chemistry, College of Agriculture, Kinki University Higashi Osaka, Osaka 577, Japan

Polyadenylated RNA was isolated from the protein-body rich fraction of developing rice (Oryza sativa. L) seeds. In a wheat germ cell-free system, the isolated polyadenylated RNA produced polypeptides with the same solubility as prolamin subunits. The electrophoretic mobility of the polypeptides suggested the presence of a signal peptide.

4To whom correspondences should be addressed

(Received May 13, 1986; Accepted July 25, 1986)
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