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Plant and Cell Physiology, 1986, Vol. 27, No. 7 1387-1393
© 1986

Article

Collection of Pure Phloem Sap from Wheat and its Chemical Composition

Hiroaki Hayashi and Mitsuo Chino

Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo Bunkyo-ku, Tokyo 113, Japan

Wheat Phloem sap was collected without contamination from the stylets of small brown planthopper severed by a YAG laser beam. The sugar, amino acid and inorganic ion composition was determined using only one µl of the sap.

The sap had a high sucrose level (251 mM), and also a high K+ level (299 mM). Total amino acid compounds in the sap reached 262 mM. The dominant amino acids were glutamic acid, aspartic acid and serine, while r-amino butylic acid was absent. The major anion in the sap was Cl– and its concentration was 25.1 mM. Nitrate was also present at a concentration of 8.1 mM.

These results suggested that the sap obtained from the cut end of the stylets of the small brown planthopper was a phloem origin of wheat.

(Received May 21, 1986; Accepted August 7, 1986)
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