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Plant and Cell Physiology, 1986, Vol. 27, No. 7 1351-1359
© 1986

Article

Intracellular pH Regulation in an Acidophilic Unicellular Alga, Cyanidium caldarium: 31P-NMR Determination of Intracellular pH

Isao Enami1, Hideo Akutsu2 and Yoshimasa Kyogoku3

1Department of Biology, Faculty of Science, Science University of Tokyo Kagurazaka, Shinjuku-ku, Tokyo 162, Japan
2Department of Physical Chemistry, Faculty of Engineering, Yokohama National University Tokiwadai, Hodogaya-ku, Yokohama 240, Japan
3Institute for Protein Research, Osaka University Suita, Osaka 565, Japan

The intracellular pH of an acidophilic unicellular alga, Cyanidium caldarium, was determined as a function of external pH by 31P nuclear magnetic resonance. The algal cells incubated under aerobic conditions or under anaerobic and illuminated conditions maintained the intracellular pH in the range from 6.8 to 7.0 even when the external pH was changed from 1.2 to 8.4. Under anaerobic and dark conditions, however, the intracellular pH acidified at the acidic pH region of the external medium. The acidified intracellular pH reversibly returned to neutral either on aeration or illumination. The results indicate that, in Cyanidium cells growing in extremely acidic environments, an active H+ efflux (H+ pump) which depends on metabolic activity (respiration or photosynthesis) is essential to maintain the intracellular pH at a constant physiological level against the passive H+ leakage due to the steep pH gradient across the cell membrane.

(Received March 19, 1986; Accepted July 17, 1986)
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