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Plant and Cell Physiology, 1986, Vol. 27, No. 7 1327-1333
© 1986

Article

NAD Kinase in Corn: Regulation by Far Red Light is Mediated by Ca2+ and Calmodulin

Peter Dieter1,2 and Dieter Marmé2,3

1Biochemical Institute, University of Freiburg Hermann Herder Strasse 7, D-7800 Freiburg, F.R.G.
2Institute of Biology III, University of Freiburg Schändestrasse 1, D-7800 Freiburg, F.R.G
3Gödecke Research Institute Mooswaldallee, 1-9, D-7800 Freiburg, F.R.

Far red light irradiation of intact corn seedlings (Zea mays L.) has neither an effect on the cellular distribution nor on the Ca2+, calmodulin-dependence of the NAD kinase (EC 2.7.1.23 [EC] ). The enzyme is located in the outer mitochondrial membrane and its activity is totally dependent on the presence of both Ca2+ and calmodulin, independently of the illumination. In intact mitochondria and the presence of calmodulin the enzyme activity increases linearly from 100 nM to 1 mM. At 100 µM Ca2+half maximal activation occurs at about 10 nM calmodulin. After solubilization and purification by calmodulin-Sepharose chromatography the Ca2+dependence of the enzyme changes. The activation reaches a plateau at about 100 µM Ca2+ and half maximal activation occurs at about 6 µM Ca2+. On the other hand irradiation of intact corn seedlings as well as an increase of the cellular Ca2+ concentration leads to an increase of NADP and a corresponding decrease of NAD. Based on these data we suggest that the light effect on the NAD kinase activity is mediated by Ca2+ and calmodulin.

(Received May 31, 1986; Accepted July 14, 1986)
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