Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Botha, F. C.
Right arrow Articles by de Vries, C.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Botha, F. C.
Right arrow Articles by de Vries, C.
Agricola
Right arrow Articles by Botha, F. C.
Right arrow Articles by de Vries, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1986, Vol. 27, No. 7 1285-1295
© 1986

Article

Isolation and Characterization of Pyrophosphate:D-Fructose-6-phosphate 1-Phosphotransferase from Cucumber Seeds

Frederik C. Botha, J.G. Chris Small and Christa de Vries

Department of Botany, University of the O.F.S. Bloemfontein 9300, South Africa

Pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase was purified over 700-fold from germinating cucumber (Cucumis sativus cv. Fletcher) seeds. The purified enzyme has a specific activity of 5.2 µmol.min–1.mg protein–1 in the presence of 1 µM fru-2,6-P2. The pH optima is similar for both the forward and reverse reactions (pH 7.5–7.8). Magnesium, manganese and cobalt activate the enzyme, with the highest affinity being for magnesium. The enzyme exhibits normal Michaelis-Menten kinetics in both the presence and absence of fru-2,6-P2. Half-maximum activation of the enzyme was obtained with 35 nM fru-2,6-P2. Fru-2,6-P2 stimulates activity by increasing Vmax and increasing the affinity for fru-6-P, fru-1,6-P2 and PPi. Phosphate causes noncompetitive inhibition with respect to both fru-6-P and PPi. On the basis of the steadystate substrate interaction and Pi inhibition data a sequential ternary complex mechanism is proposed.

(Received April 28, 1986; Accepted July 9, 1986)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.