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Plant and Cell Physiology, 1986, Vol. 27, No. 7 1249-1254
© 1986

Article

The Effect of Short-Term H2S Fumigation on Nitrate Reductase Activity in Spinach Leaves

Luiet J. De Kok, Ineke Stulen, Wiebe Bosma and Jikkie Hibma

Department of Plant Physiology, University of Groningen P.O. Box 14, 9750 AA Harm, The Netherlands

Short-term exposure of spinach plants to 250 ppb H2S at a photon fluence rate of 35µmol m–2s–1 (within the 400–700 nm range) in the ambient air did not affect in vitro nitrate reductase activity (NRA) in the leaves. Likewise, H2S exposure did not significantly affect in vivo NRA measured under anaerobic conditions. In vivo NRA of untreated plants was apparently inhibited in the presence of oxygen. However, shortterm H2S exposure increased in vivo "aerobic" NRA up to five fold of that of untreated plants. H2S induced increase of in vivo "aerobic" NRA depended on the sulfide concentration. After 24 hours of exposure maximal increase (two to five fold) of in vivo NRA "aerobic" was observed at 220 ppb H2S. It is proposed that H2S inhibited NADH oxidizing enzymes, which resulted in an increase in NADH supply to nitrate reductase (NR) in the presence of oxygen. It was unlikely that the increase in in vivo "aerobic" NRA in sulfide exposed plants was due to an altered competition between mitochondrial respiration and NR since leaf respiration was not affected by an exposure to 250 ppb H2S

(Received February 12, 1986; Accepted June 27, 1986)
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