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Plant and Cell Physiology, 1986, Vol. 27, No. 6 1201-1204
© 1986

Short Communication

Cell-Free Synthesis of the Rice Glutelin Precursor

Masakazu Furuta1, Hiroshi Yamagata2, Kunisuke Tanaka2,4, Zenzaburo Kasai3 and Shoji Fujii2

1The Central Institute for Radiation, Osaka Prefecture Sakai, Osaka 593, Japan
2Department of Agricultural Chemistry, College of Agriculture, Kyoto Prefectural University Shimogamo, Sakyo, Kyoto 606, Japan
3Department of Agricultural Chemistry, College of Agriculture, Kinki University Higashi Osaka, Osaka 577, Japan

4To whom correspondences should be addressed.

Polyadenylated RNA was directly isolated from developing rice (Oryza sativa. L) seeds. The major translation product of the isolated polyadenylated RNA in a rabbit reticulocyte cell-free system was about 2 kDa larger than the in vivo labeled 57 kDa glutelin precursor, and was immunoprecipitated by antiserum against the glutelinprolamin fraction of mature seeds.

(Received January 27, 1986; Accepted June 23, 1986)
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