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Plant and Cell Physiology, 1986, Vol. 27, No. 6 1191-1195
© 1986

Short Communication

Effects of Calcium on Template Activity and Size of Pea Chromatin Fragments

Hideaki Matsumoto

Institute for Agricultural and Biological Sciences, Okayama University Chuo 2-20-1, Kurashiki, Okayama 710, Japan

Pea chromatin was fractionated into template active and inactive fragments by cleavage with DNase II followed by fractionation with Bio-Gel A 5 m column chromatography.

When the chromatin fragments were chromatographed in the presence of Ca2+ or EGTA, the elution patterns suggested that Ca2+ assembled moderately digested chromatin fractions. Moreover, total template activity of chromatin fractions was reduced by Ca2+ and the active fraction was eluted at different positions under the chelated and non-chelated conditions.

(Received February 12, 1986; Accepted May 28, 1986)
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