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Plant and Cell Physiology, 1986, Vol. 27, No. 6 1069-1080
© 1986

Article

Changes in mRNA of Vigna mungo Cotyledons during Seed Germination

Tomokazu Koshiba, Hideaki Tomura and Masayuki Miura1

Department of Biology, Tokyo Metropolitan University Setagayaku, Tokyo 158, Japan

Quantitative and qualitative changes of mRNA in Vigna mungo cotyledons during seed germination have been investigated. Total RNA is higher in dry cotyledons and declines during germination. Poly(A)+ RNA also is present at a relatively high level in dry cotyledons, increases slightly during the first day of germination, and then decreases. Polysomal RNA is very low in dry cotyledons but increases rapidly during the first day of germination, and then declines. The translational activity of the mRNA in a wheat germ cell-free system is low on day 0 but increases rapidly on day 1 of germination. Two-dimensional gel electrophoresis of in vitro translation products reveals that many new peptides are synthesized on day 1 of germination. Synthesis of most of these polypeptides continue throughout 5 days of germination.

Change in the mRNA population during germination has been investigated using cDNA against poly(A)+ RNA from 3-day-old cotyledons. With total RNA of day 3 and 5, the cDNA strongly hybridized with RNA similar in size to 25 S ribosomal RNA, but no specific bands are detected with samples of day 0 or 1. With poly(A)+ RNA of day 5 or 1, the cDNA tends to hybridize with RNAs of relatively small molecular size. Cordycepin and {alpha}-amanitin prevent the increase in poly (A)+ RNA content and the appearance of new mRNAs during the first day of germination.

1Present address: Division of Regulation of Macromolecular Function, Institute for Protein Research, Suita City, Osaka 565, Japan.

(Received January 13, 1986; Accepted June 10, 1986)
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