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Plant and Cell Physiology, 1986, Vol. 27, No. 6 1033-1041
© 1986

Article

In vivo Studies on Protein Synthesis in Developing Seeds of Canavalia gladiata D.C.

Daisuke Yamauchi and Takao Minamikawa

Department of Biology, Tokyo Metropolitan University Fukazawa, Setagaya-ku, Tokyo 158, Japan

In successive stages of seed development of Canavalia gladiata, fruits were harvested and time-course changes of seed protein accumulation and SDS-gel electrophoretic patterns of the polypeptides were examined. Albumin content increased gradually after the stage of 1.8 g fresh weight per seed, reached a peak, then decreased at later stages. SDS-gel electrophoresis showed a clear change in albumin composition between the stages of 2.3 g and 3.6 g fresh weight per seed. Seed globulins were synthesized actively after the stage of 1.8 g fresh weight per seed, and the major polypeptides of seed globulins, including the main polypeptides of canavalin, were first detected at this stage by gel electrophoresis. In contrast to the albumins, these polypeptides of globulins continued to accumulate until the maturation was completed. In vivo protein synthesis was also analyzed by SDS-gel electrophoresis and fluorography, after [35S]methionine had been fed to cotyledon sections of maturing seeds at the stages of 3.4 g and 5.0 g fresh weight per seed. The results indicated that the synthesis of canavalin starts earlier than that of concanavalin A and also ends earlier during seed development.

(Received December 10, 1985; Accepted June 2, 1986)
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