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Plant and Cell Physiology, 1986, Vol. 27, No. 5 819-835
© 1986

Article

Lipid-Linked Desaturation of Palmitic Acid in Monogalactosyl Diacylglycerol in the Blue-Green Alga (Cyanobacterium) Anabaena variabilis Studied in Vivo

Naoki Sato1,4, Yousuke Seyama2,5 and Norio Murata3

1Department of Biology, College of Arts and Sciences, University of Tokyo Komaba, Tokyo 153, Japan
2Department of Biochemistry, Faculty of Medicine, University of Tokyo Hongo, Tokyo 113, Japan
3Department of Regulation Biology, National Institute for Basic Biology Okazaki 444, Japan

The mechanism of desaturation of palmitic acid in monogalactosyl diacylglycerol in Anabaena variabilis was studied by labeling in vivo with 13C and mass spectrometry. When the cells were fed with [13C]Na2CO3 for 2.5 h, 19% of the palmitic, but virtually none of the palmitoleic, acid at the C-2 position of the lipid was enriched with 13C. During subsequent incubation for 7.5 h, the [13C]palmitic acid was desaturated to [13C]palmitoleic acid. Mass spectrometric analysis of the 2-acylglycerol moiety of the lipid indicated that [13C]palmitoyl-[13C]glycerol and [12C]palmitoyl-[12C]glycerol were converted to [13C]palmitoleoyl-[13C]glycerol and [12C]palmitoleoyl-[12C]glycerol, respectively. These results suggest that the palmitic acid was converted to palmitoleic acid in vivo by lipid-linked desaturation but not via a pathway consisting of deacylation, desaturation and reacylation.

4Present address: Department of Botany, Faculty of Science, University of Tokyo, Hongo, Tokyo 113, Japan

5Present address: Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Hongo, Tokyo 113, Japan

(Received December 7, 1985; Accepted April 16, 1986)
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