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Plant and Cell Physiology, 1986, Vol. 27, No. 5 785-790
© 1986

Article

Effect of 5-Fluorouracil on Growth and Morphogenesis of Tissue Cultures of Nicotiana sylvestris

Francesco Maria Restivo and Francesca Tassi

Institute of Genetics, University of Parma 43100 Parma, Italy

Tissue cultures of Nicotiana sylvestris treated with increasing concentration of 5-fluorouracil (5-FU) showed a differential effect of the drug on growth and morphogenesis. In the range of concentrations tested, 5-FU did not inhibit increases in fresh weight, but it inhibited the production of shoots. The effect of 5-FU on shoot production was reversible and depended on the time of adding 5-FU to the tissue culture. Uracil and thymine did not counteract the effect of 5-FU on morphogenesis, whereas uracil partially reduced the inhibition of growth under some culture conditions.

(Received July 16, 1985; Accepted April 5, 1986)
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