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Plant and Cell Physiology, 1986, Vol. 27, No. 5 755-763
© 1986

Article

Metabolism and Biosynthesis of Cytokinins in Tobacco Crown Gall Cells

Hiromitsu Nakajima1, Takao Yokota and Nobutaka Takahashi

Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan
1Department of Agricultural Chemistry Tottori University, Koyama, Tottori 680, Japan.

The metabolism of ribosylzeatin (RZ) was studied using tobacco crown gall cells which produce RZ as one of the major endogenous cytokinins. When [8-14C]RZ was fed to the cells, it was converted into its phosphate (which was rigorously determined to be the 5'-monophosphate), RZ-O-glucoside, inosine (or its phosphate), adenosine and adenosine-O-glucoside. When [8-14C]N6-({Delta}2-isopentenyl)adenosine (i6Ado), a probable precursor of RZ, was fed to the cells, it was converted into (i6Ado)-O-glucoside, inosine (or its phosphate), adenosine, adenosine-O-glucoside and adenosine phosphate, but no incorporation of radioactivity into RZ was observed. The present study led to the following conclusions: i) i6Ado is not a precursor of RZ in the cells, ii) both deaminase and cytokinin oxidase are involved in the catabolism of cytokinin, and iii) the metabolism of RZ is quite different from that of i6Ado.

(Received December 24, 1985; Accepted April 1, 1986)
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