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Plant and Cell Physiology, 1986, Vol. 27, No. 4 741-744
© 1986

Short Communication

Isolation of Chloroplast DNA from Pinus

Teiji Kondo1,3, Takashi Ishibashi2, Masaru Shibata2 and Atsushi Hirai1

1Graduate Division of Biochemical Regulation, Faculty of Agriculture, Nagoya University Nagoya 464, Japan
2Kameyama Breeding Station, Oji Institute of Forest Tree Improvement, Oji Paper Co., Ltd. Kameyama, Mie 519-02, Japan

Chloroplast DNA isolated from Japanese black pine [Pinus thunbergii) was digested with three restriction endonucleases. The size of this DNA was estimated to be 120 kbp. Chloroplast DNA from Japanese red pine (P. densiflora) could be distinguished from that of P. thunbergii by BamHI restriction patterns.

3Present address: Kanto Forest Tree Breeding Institute, Kasahara, Mito, Ibaraki 310, Japan.

(Received December 10, 1985; Accepted March 8, 1986)
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