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Plant and Cell Physiology, 1986, Vol. 27, No. 4 635-643
© 1986

Article

Stimulation of RNA Synthesis in Isolated Nuclei by Auxin-Binding Proteins-I and -II

Shingo Sakai1, Junichi Seki1 and Hidemasa Imaseki2

1Laboratory of Cell Biochemistry, Faculty of Science, Saitama University Urawa 338, Japan
2Research Institute for Biochemical Regulation, Faculty of Agriculture, Nagoya University Nagoya 464, Japan

The auxin-binding proteins (ABP-I and ABP-II) purified from etiolated mung bean seedlings stimulated RNA synthesis in isolated nuclei both in the presence and absence of (NH4)2SO4. In the absence of (NH4)2SO4, maximum stimulation of RNA synthesis occurred at 100 µg ABP-I (25%) and 300 µg ABP-II (60%), whereas in the presence of 50 mM (NH4)2SO4 maximum stimulation occurred at 60 µg ABP-I (10%) and 100 µg ABP-II (40%). These stimulatory effects on RNA synthesis by ABP-I and ABP-II were completely abolished by the addition of {alpha}-amanitin (4 µg/0.5 ml reaction mixture). IAA had no effect on the stimulation of RNA synthesis by ABP-I and ABP-II.

(Received September 30, 1985; Accepted March 5, 1986)
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