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Plant and Cell Physiology, 1986, Vol. 27, No. 4 627-633
© 1986

Article

Immunological Characterization of Nitrate Reductase in Different Tissues of Spinach Seedlings

Hiroki Nakagawa1, Kenji Yamagishi1, Naoko Yamashita1, Takahide Sato1, Nagao Ogura1 and Ann Oaks2

1Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University Matsuda, Chiba 271, Japan
2Department of Biology, McMaster University Hamilton, Ontario, Canada L8S 4K1

In spinach seedlings and roots, NADH-nitrate reductase (NR) activity (per g fresh weight) decreased as the seedlings aged. Experiments using double immunodiffusion analysis and immunotitration showed no differences in the immunological properties of NR from spinach seedlings at various stages of aging. Comparison of spinach leaf to the spinach root enzyme using the Ouchterlony double diffusion technique revealed a high degree of similarity between them.

(Received November 6, 1985; Accepted March 4, 1986)
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