Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Okada, K.
Right arrow Articles by Takebe, I.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Okada, K.
Right arrow Articles by Takebe, I.
Agricola
Right arrow Articles by Okada, K.
Right arrow Articles by Takebe, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1986, Vol. 27, No. 4 619-626
© 1986

Article

Introduction of Functional RNA into Plant Protoplasts by Electroporation

Kazuya Okada1, Toshiyuki Nagata2 and Itaru Takebe1

1Department of Biology, Faculty of Science, Nagoya University Furo-cho, Chikusa-ku, Nagoya 464, Japan
2Department of Cell Biology, National Institute for Basic Biology 38 Nishigonaka, Myodaiji-cho, Okazaki 444, Japan

A simple apparatus was constructed for producing electric discharge of varying intensities between two electrodes in a spectrophotometer cuvette. This apparatus was used to study the conditions for efficient introduction of functional RNA into plant protoplasts using RNAs of tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV). Electroporation under optimal conditions resulted in the production of TMV and CMV in 80% of the protoplasts from tobacco cell line BY2. Infection by TMVRNA occurred in 60% of Vinca rosea suspension culture protoplasts and in 40% of tobacco mesophyll protoplasts. Electroporation in the presence of TMV and CMV particles also resulted in infection, suggesting that pores larger than 30 nm are formed. The advantages and uses of the electrical method for introduction of functional RNA are discussed.

(Received December 21, 1985; Accepted March 1, 1986)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
T. Suzuki, S. Kawano, A. Sakai, M. Fujie, H. Kuroiwa, H. Nakamura, and T. Kuroiwa
Preferential mitochondrial and plastid DNA synthesis before multiple cell divisions in Nicotiana tabacum
J. Cell Sci., November 1, 1992; 103(3): 831 - 837.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.