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Plant and Cell Physiology, 1986, Vol. 27, No. 4 601-606
© 1986

Article

Effects of Nutrient Limitation and {gamma}-Irradiation on Tracheary Element Differentiation and Cell Division in Single Mesophyll Cells of Zinnia elegans

Munetaka Sugiyama1,1, Hiroo Fukuda2 and Atsushi Komamine3

1Department of Botany, Faculty of Science, University of Tokyo Hongo, Tokyo 113, Japan
2Department of Biology, Faculty of Science, Osaka University Toyonaka, Osaka 560, Japan
3Biological Institute, Faculty of Science, Tohoku University Sendai, Miyagi 980, Japan

The effects of nutrient limitation and {gamma}-irradiation on tracheary element differentiation and cell division were investigated using single cells isolated from the mesophyll of Zinnia elegans. When the phosphate concentration of the medium was reduced to 10 µM (1/50 of Fukuda and Komamine's medium, 1980a), the frequency of cell division during 4 days of culture decreased, while the frequency of tracheary element differentiation was unaffected. {gamma}-Irradiation with a dose of 92 Gy at 36 h of culture preferentially and thoroughly suppressed cell division without reducing the number of tracheary elements formed. The appearance of secondary cell wall thickenings was delayed by irradiation, but synchrony was maintained. Thus the Zinnia system previously reported [Fukuda and Komamine (1980a) Plant Physiol. 65: 57] was improved to give a more useful system for the study of cytodifferentiation, in which tracheary element formation occurred from single cells without cell division.

1Present address: Biological Institute, Faculty of Science, Tohoku University, Sendai, Miyagi 980, Japan.

(Received November 28, 1985; Accepted February 22, 1986)
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