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Plant and Cell Physiology, 1986, Vol. 27, No. 3 443-452
© 1986

Article

Nitrogen-Regulated Accumulation of mRNA and Protein for Photosynthetic Carbon Assimilating Enzymes in Maize

Masanori Yamazaki1, Akira Watanabe and Tatsuo Sugiyama2

School of Agriculture, Nagoya University Chikusa, Nagoya 464, Japan

2To whom correspondence should be addressed.

Nitrogen and light effects on kinetic patterns of mRNA and protein accumulations for ribulose 1,5-bisphosphate carboxylase (RuBPC), phosphoenolpyruvate carboxylase (PEPC), and pyruvate orthophosphate dikinase (PPDK) were examined by analyzing the transient states induced by supplementing nitrogen or light intensity to Zea mays plants grown for 3 weeks under low nitrate (0.8 nM KNO3) and/or low light (12% full sunlight) conditions. When plants grown previously under low nitrate were supplemented with high nitrate (16 mM KNO3) for 3 days under high sunlight, mRNAs for these enzymes accumulated progressively as did their proteins. The accumulation of mRNA for these enzymes was stimulated in plants previously grown under high nitrate by increasing light intensity from low light to high light (48% full sunlight). This stimulation, however, did not occur in plants previously grown under low nitrate. These results imply that nitrogen is a regulatory factor essential for the light stimulation of mRNA accumulation of these enzymes. The kinetic patterns of protein and mRNA accumulation also indicate that the accumulation of proteins of PEPC and PPDK is a reflect of the level of respective mRNA, irrespective of the conditions used for inducing transient changes in gene expression. For RuBPC, protein and mRNA accumulations did not necessarily coincide.

1Present address: Laboratories of Biology and Pharmacology, Research Laboratories, Nippon Kayaku Co., Ltd., Shimo 3-31-12, Kita-ku, Tokyo 115, Japan.

(Received September 26, 1985; Accepted January 22, 1986)
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