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Plant and Cell Physiology, 1986, Vol. 27, No. 2 331-335
© 1986

Article

Mechanism of the Increase in Ribonuclease Activity in Potato Tuber Slices

Maria Clara Isola and Luis Franzoni

Departmento de Biologia Molecular, Facultad de Ciencias Exactas, Fisico-Quimicas y Naturales, Universidad Nacional de Rio Cuarto Estafeta Postal No. 9, 5800 - Rio Cuarto - (Cordoba), Argentina

Upon wounding of potato tubers (Solanum tuberosum L. cv. Spunta) RNase activity increases, peaks in about 16 hours, then declines. To see if the increase of the activity is due to de novo synthesis of the enzyme protein, the extracts were compared for their ability to react with a rabbit antibody prepared against the wound activated RNase. The enzyme was purified by polyacrylamide gel electrophoresis of a RNase preparation, which had been partially purified from aged potato slices by ammonium sulfate precipitation, carboxymethyl-Sephadex column chromatography and gel filtration through Sephadex G-100. Using rocket immunoelectrophoresis RNase-protein immunoprecipitated by the antibody increased in wounded tissue. This observation implies that the activity increase involves enzyme synthesis. The increase was inhibited by actinomycin D and cordycepin, but not by 5-fluorouracil, suggesting a requirement for mRNA synthesis.

(Received April 9, 1985; Accepted December 16, 1985)
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