Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hoson, T.
Right arrow Articles by Wada, S.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Hoson, T.
Right arrow Articles by Wada, S.
Agricola
Right arrow Articles by Hoson, T.
Right arrow Articles by Wada, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1986, Vol. 27, No. 2 303-309
© 1986

Article

N-Acetylglucosamine-Containing Glycopeptide Released from Rice Coleoptile Cell Walls by Protease Treatment

Takayuki Hoson1,3 and Shunji Wada2

1Department of Biology, Osaka Kyoiku University Tennoji-ku, Osaka 543, Japan
2Biological Institute, Faculty of Science, Tohoku University Aoba-yama, Sendai 980, Japan

N-Acetylglucosamine-containing glycopeptides were released from the cell walls of rice coleoptiles by treatment with subtilisin. They were purified by successive treatments with different types of proteases and by affinity chromatography using wheat germ lectin- and concanavalin A-Sepharose columns. The glycopeptide finally obtained after gel filtration contained glycine as the N-terminal amino acid and asparagine as the only amino acid capable of linking with the sugar residue. This glycopeptide contained only N-acetylglucosamine and mannose as sugars and could be hydrolyzed by {alpha}-mannosidase and by almond glycopeptidase. It seems to have an oligosaccharide structure, consisting of {alpha} and ß-mannose and chitobiose attached to asparagine. The results indicate that this wall glycopeptide is a component of asparagine-linked glycoprotein.

3Present address: Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, Osaka 558, Japan.

(Received May 22, 1985; Accepted December 10, 1985)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
R. Zhong, S. J. Kays, B. P. Schroeder, and Z.-H. Ye
Mutation of a Chitinase-Like Gene Causes Ectopic Deposition of Lignin, Aberrant Cell Shapes, and Overproduction of Ethylene
PLANT CELL, January 1, 2002; 14(1): 165 - 179.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.