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Plant and Cell Physiology, 1986, Vol. 27, No. 2 253-260
© 1986

Article

Effect of Inhibitors of Polyamine Biosynthesis on Gibberellin-Induced Internode Growth in Light-Grown Dwarf Peas1

Ravindar Kaur-Sawhney, Yao-ren Dai2 and Arthur W. Galston

Department of Biology, Yale University New Haven, Connecticut 06511, U.S.A.

When gibberellic acid (GA3) is sprayed on 9-day-old light-grown dwarf Progress pea (Pisum sativum) seedlings, arginine decarboxylase (ADC; EC 4.1.1.9 [EC] ) activity increases within 3 h and peaks at about 9 h after GA3 application. This is followed by a second lower peak at about 30 h; both peaks were higher than the corresponding peaks in the controls. In contrast, no appreciable effect of GA3 on internode length was observed until about 12 h, after which time a dramatic increase in growth rate occurred and persisted for about 12 h. Specific (DL-{alpha}-difluoromethylarginine) and non-specific (Darginine and L-canavanine) inhibitors of ADC strongly inhibited ADC activity and to a lesser extent internode growth. The inhibition was reversed only slightly by the addition of polyamines. Actinomycin D and cycloheximide inhibited the rise in ADC activity induced by GA3. The half-life of the enzyme was increased by GA3 treatment.

The results suggest that part of the GA3-induced increase in internode growth may result from enhanced polyamine biosynthesis through the ADC pathway. Furthermore, the GA3 induced increase in ADC activity probably requires de novo synthesis of both RNA and protein.

1 Supported by grants from the National Science Foundation and Binational Agricultural Research and Development Fund to A.W.G.

2Present address: Department of Biology, Peking University, Beijing, People's Republic of China.

(Received September 9, 1985; Accepted November 27, 1985)
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