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Plant and Cell Physiology, 1986, Vol. 27, No. 2 243-251
© 1986

Article

2,4-D Resistance in a Tobacco Cell Culture Variant II. Effects of 2,4-D on Nucleic Acid and Protein Synthesis and Cell Respiration

Chiharu Nakamura, Naoki Mori, Masanobu Nakata and Hajime Ono

Department of Plant Protection, Faculty of Agriculture, Kobe University Kobe 657, Japan

The effects of 2,4-D on nucleic acid and protein synthesis and cell respiration were compared between a 2,4-D-resistant variant and its wild-type cell lines of tobacco (Nicotiana tabacum L.). The variant continued cell division and growth in the presence of 100 µM 2,4-D which was strongly inhibitory to the wild-type cell lines. Among the macromolecular syntheses studied, DNA synthesis was the most sensitive and protein synthesis was the least sensitive to inhibitory concentrations of 2,4-D. The variant displayed threefold higher resistance to 2,4-D than the wild-type cell line based on the 50% inhibitory concentrations of 2,4-D on DNA synthesis. No significant differences which could explain the 2,4-D resistance were found between the variant and the wild-type cell lines in 2,4-D concentrations required to inhibit RNA and protein synthesis. The effect of 2,4-D on cell respiration was detectable without a noticeable lag. The resistance of the variant based on the effect on cell respiration also was apparent immediately after 2,4-D addition. According to the 50% inhibitory concentrations of 2,4-D on cell respiration, the variant showed a level of resistance similar to that estimated by DNA synthesis. These results indicate that the resistance of the variant is due to a modification which reduces the cellular sensitivity to phyto-toxic concentrations of 2,4-D with respect to, at least, DNA synthesis and respiration.

(Received August 6, 1985; Accepted November 27, 1985)
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