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Plant and Cell Physiology, 1986, Vol. 27, No. 1 91-101
© 1986

Article

Calcium-Sensitivity of Cortical Microtubules in the Green Alga Mougeotia

Tatsuo Kakimoto and Hiroh Shibaoka

Department of Biology, Faculty of Science, Osaka University Toyonaka 560, Japan

Membrane ghosts were prepared from protoplasts of the green alga Mougeotia, and the Ca2+-sensitivity of microtubules on the ghosts was examined.

Microtubules on the protoplast ghosts were not depolymerized by 3 min treatment with 1 mM Ca2+. As the treatment was prolonged, some depolymerization of microtubules became evident, but even after 10 min about 50% of the ghosts showed no depolymerization. Ca2+ introduced into intact protoplasts seemed to be ineffective in depolymerizing microtubules; abundant microtubules were present on membrane ghosts prepared from protoplasts which had been treated with 2x10–5M Ca2+-ionophore A23187 [GenBank] plus 1 mM Ca2+ for 20 or 30 min.

Neither 3 min treatment with 0.2% Triton X-100 nor with 1 mM Ca2+ solution containing 5 min MgSO4 and 100 mM KCl caused depolymerisation of microtubules on protoplast ghosts. However, when given successively, these treatments caused complete depolymerization of microtubules.

These results suggest that Mougeotia microtubules are stable to Ca2+ and that the stability is conferred by a microtubule-associated factor which can easily be removed by Triton X-100 treatment.

(Received July 19, 1985; Accepted October 25, 1985)
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