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Plant and Cell Physiology, 1986, Vol. 27, No. 1 61-66
© 1986

Article

Callus Formation, Plant Regeneration and Clonal Propagation in Vitro of Gynura aurantiaca (Blume) DC.

Manuel V. Roche1, Luis A. Roig1 and Vicente Moreno2

1Department of Microbiology, E.T.S. Ingenieros Agrónomos, Universidad Politécnica de Velencia Spain
2Department of Genetics, E.T.S. Ingenieros Agrónomos, Universidad Politécnica de Valencia Spain

Methods for culture in vitro of Gynura aurantiaca (Blume) DC. are described. Callus formation from gynura explants was initially difficult in spite of the multiple combinations of plant growth regulators assayed. In order to induce high growth rate, incorporation of several organic addenda to a basal medium was necessary, the best of these being coconut milk. Unorganized cell lines could be obtained by subculturing on C medium supplemented with 10% coconut milk, and transfers had to be done at short intervals.

A morphogenetic cell line without apparent loss in its organogenic potential through more than 8 months of subculturing was established following a sequence of culture media.

Methods for rapid and efficient clonal propagation in vitro of this plant species are also reported.

The possible utilization of these tissue culture techniques developed for gynura, a suitable indicator host for Citrus Exocortis Viroid, in further studies concerning pathogenesis and replication of this viroid is discussed.

(Received April 22, 1985; Accepted October 11, 1985)
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