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Plant and Cell Physiology, 1986, Vol. 27, No. 1 37-47
© 1986


Article

Kinetic Studies on the Active Species of Inorganic Carbon Absorbed by the Cells of Dunaliella tertiolecta

Katsunori Aizawa, Mikio Tsuzuki and Shigetoh Miyachi

Institute of Applied Microbiology, University of Tokyo Bunkyo-ku, Tokyo 113, Japan

Cells of Dunaliella tertiolecta which had been grown in ordinary air (low-CO2 cells) had high carbonic anhydrase (CA) activity on the cell surface and mainly utilized HCO3 for photosynthesis. When CA activity on the cell surface was inhibited by Diamox or subtilisin, the cells utilized CO2. When bovine CA was added, the subtilisin-treated low-CO2 cells utilized mainly HCO3. When grown in air containing 2% CO2, the cells had low CA activity on the cell surface, and preferred CO2 to HCO3. Kinetic analysis of these results indicated that low-CO2 cells of D. tertiolecta absorb CO2 which was converted from HCO3 via the CA located on the cell surface.

(Received June 29, 1985; Accepted October 9, 1985)
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