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Plant and Cell Physiology, 1986, Vol. 27, No. 1 25-36
© 1986

Article

Characteristics of the Cl– Action Site in the O2 Evolving Reaction in PS II Particles: Electrostatic Interaction with Ions

Shigeru Itoh and Sachiko Uwano

National Institute for Basic Biology Nishigonaka 38, Myodaijicho, Okazaki 444, Japan

The role of Cl– in the reactivation of O2 evolution in photosystem II (PS II) particles derived from spinach chloroplasts was studied in the presence of various salts. Multivalent ion (especially anion) salts were found to strongly suppress the reactivation of O2 evolution by Cl– in the Cl–-depleted PS II particles in a competitive manner. The effectiveness of anions in the suppression of Cl–-supported O2 evolution was in the order of trivalent>divalent>monovalent ones. Multivalent anions similarly suppressed O2 evolution in the untreated PS II particles under low and moderate Cl– concentrations. pH dependence of the Cl–-affinity (Km) value for Cl–) was also studied. Within the pH range 5.5 to 8 the Km value became higher as the pH of the medium increased. These results suggest that the membrane surface in the vicinity of the Cl– action site is net positively charged and attracts Cl– electrostatically, and that the site is almost freely accessible to various anions. The origin and role of the local net positive domain and the role of peripheral proteins are discussed.

(Received May 27, 1985; Accepted October 8, 1985)
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