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Plant and Cell Physiology, 1986, Vol. 27, No. 1 183-186
© 1986

Short Communication

Partial Enrichment of Pea Phytochrome mRNA by Gel Electrophoresis and Transfer to Poly (U)-Paper

Naoki Sato1, Ken-ichi Tomizawa1, Yoshibumi Komeda2 and Masaki Furuya1,3

1Department of Botany, Faculty of Science, University of Tokyo Hongo, Tokyo 113, Japan
2Molecular Genetics Research Laboratory, University of Tokyo Hongo, Tokyo 113, Japan
3Department of Biological Regulation, National Institute for Basic Biology Okazaki 444, Japan

Total cellular poly(A)+RNA from etiolated pea epicotyls was fractionated by urea-agarose gel electrophoresis and recovered by transfer to poly(U)-paper. Phytochrome mRNA activity was detected in RNA fractions that migrated slightly slower than the 25S rRNA in the electrophoresis. A single run of this fractionation enriched the phytochrome mRNA content 20-fold. This technique together with hybridization on poly(U)-paper made it possible to compare the mobilities of translatable and hybridizable phytochrome mRNA. The advantages of the present method of mRNA fractionation in preparing enriched mRNA and identifying cDNA clone are discussed.

(Received September 28, 1985; Accepted November 8, 1985)
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