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Plant and Cell Physiology, 1986, Vol. 27, No. 1 147-153
© 1986

Article

Induction of Cell Division Synchrony and Variation of Cytokinin Contents through the Cell Cycle in Tobacco Cultured Cells

Noriko Nishinari1 and Kunihiko Syono

Department of Pure and Applied Sciences, College of Arts and Sciences, University of Tokyo Komaba, Meguro-ku, Tokyo 153, Japan

Cell division synchrony was induced in tobacco {Nicotiana tabacum) cultured cells by several treatments. Very high synchrony throughout two cell cycles was induced by aphidicolin treatment (inhibitor of DNA polymerase {alpha}, 10 µg/ml) and by treatment with low temperature (4°C) and hydroxyurea (50 µg/ml). The mitotic index reached its maximum (52% and 40% in aphidicolin and hydroxyurea treatments, respectively) at 11 h after removal of the added chemical. During the treatments, the cells were arrested in the G1/S phase of the cell cycle. In the aphidicolin-induced system, incorporation of 14C-thymidine confirmed that DNA synthesis was started immediately after removal of the chemical.

The aphidicolin-induced synchronous cells were used to study the contents of butanol-soluble cytokinins during the cell cycle. Cytokinin contents increased conspicuously at the G2/M boundary.

1Present address: Department of Biology, Otsuma Women's University, Chiyodaku, Tokyo 102, Japan.

(Received May 14, 1985; Accepted November 8, 1985)
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