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Plant and Cell Physiology, 1985, Vol. 26, No. 8 1613-1616
© 1985

Short Communication

Ammonia Content in Mitochondria Isolated from Detached Pea Shoots. Effect of Light and Inhibitors of Photorespiratory Nitrogen Cycle

Tomoyuki Yamaya and Hideaki Matsumoto

Institute for Agricultural and Biological Sciences, Okayama University Chuo 2-20-1, Kurashiki, Okayama 710, Japan

Mitochondria isolated from detached pea shoots which had been grown in water for 1.5 h in the dark contained about 1.64 nmol NH4$ per mg of protein. Light treatment caused approximately 50% increase in the mitochondrial NH4$. Methionine sulfoximine slightly reduced the mitochondrial NH4$, although total NH4$ in the shoot extracts was greatly increased by the inhibitor.

(Received June 17, 1985; Accepted September 24, 1985)
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