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Plant and Cell Physiology, 1985, Vol. 26, No. 8 1565-1572
© 1985


Article

Stage-Specific Changes in Calcium-Regulated Protein Phosphorylation in Developing Tomato Fruits1,2

K. G. Raghothama, K. Veluthambi and B. W. Poovaiah3

Department of Horticulture and Landscape Architecture, Washington State University Pullman, WA 99164-6414, U.S.A.

3 To whom requests for reprints should be addressed

The role of calcium and calmodulin in the in vitro phosphorylation of soluble and membrane proteins was studied in relation to growth and development of tomato fruits. Calcium at micromolar concentrations promoted the phosphorylation of both soluble and membrane proteins. The calmodulin antagonists, chlorpromazine and trifluoperazine, inhibited the phosphorylation of several proteins. Qualitative changes were observed in the pattern of protein phosphorylation at different developmental stages. There was a general decrease in protein phosphorylation towards ripening. These results indicated that calcium may be involved in the regulation of phosphorylation of different proteins at different stages of fruit development.

1 Scientific Paper No. 7149, College of Agriculture and Home Economics, Washington State University, Pullman, Project 0321.

2 Supported in part by a grant from the National Science Foundation. DCB-8502215.


(Received May 14, 1985; Accepted September 12, 1985)
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