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Plant and Cell Physiology, 1985, Vol. 26, No. 8 1485-1491
© 1985

Article

Effect of O2 Concentration on Dark H2 Oxidation in Whole Cells of a Marine Sulfur Photosynthetic Bacterium (Chromatium sp. Strain Miami PBS 1071)

Shuzo Kumazawa1,2,3,4, Teruo Ogawa1, Yorinao Inoue1 and Akira Mitsui2

1 Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN) Wako-shi, Saitama 351-01, Japan
2 School of Marine and Atomospheric Science, University of Miami Florida 33149, U.S.A.

3 To whom correspondences should be addressed

Whole cells of photoanaerobically grown Chromatium sp. strain Miami PBS 1071, a marine purple sulfur bacterium, oxidized H2 in the dark through the oxyhydrogen reaction. Oxidation of H2 was measured by injecting either H2 into an air-equilibrated cell suspension (microaerobic H2 oxidation) or O2 into an H2/Ar-equilibrated cell suspension (microaerobic H2 oxidation). Both types of H2 oxidation were strongly inhibited by azide (40 mM), indicating that the oxidation proceeds via a terminal oxidase system. 2,5-Dibromo-3-methyl-6-isopropyl-p-benzoquinone (16 µM) inhibited aerobic H2 oxidation by 49% but it accelerated microacrobic H2 oxidation. The sensitivity of H2 oxidation to rotenone was higher under aerobic conditions. The results indicate that H2 oxidation proceeds via two different pathways; one contains ubiquinone and NAD, and the other does not. The contribution of each pathway depends on the O2 partial pressure.

4 Present address: Institute of Oceanic Research and Development, Tokai University, Shimizu, Shizuoka 424, Japan.

(Received May 24, 1985; Accepted August 29, 1985)
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