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Plant and Cell Physiology, 1985, Vol. 26, No. 8 1443-1453
© 1985

Article

Assembly of Cellulose Synthesizing Complexes on the Plasma Membrane of Boodlea coacta

Shun Mizuta

Department of Biology, Faculty of Science, Kochi University Kochi 780, Japan

The assembly of cellulose synthesizing complexes (terminal complexes, TCs) on the plasma membrane of Boodlea coacta was investigated during the formation of both the matrix-rich layer (MRL) and fibril-rich layers (FRLs) of cell walls. The TCs appeared to be located mostly within the outer leaflet of the plasma membrane, and were observed as elliptical protrusions consisting of many particles of about 9 nm in diameter. Their length varied from 100 to 500 nm (average, 220 nm) during MRL formation and from 100 to 860 nm (average, 360 nm) during FRL formation. A correlation was found between the length of TCs and the microfibril width in both MRL and FRL.

On the E-face of the plasma membrane, numerous round protrusions (30–130 nm in diameter), consisting of many particles, 8–10 nm in diameter, were also present. Their density was greater during FRL formation than during MRL formation. Some of these structures larger than 100 nm were associated with microfibril impressions and some appeared to be bound to the TCs. These protrusions increased in number with Calcofluor treatment but decreased in number when the dye was removed from the culture medium. Thus, the TCs may be assembled from masses of particles aggregated on the outer surface of the plasma membrane, and may grow longer by incorporation of these masses. The appearance of the longer TCs during FRL formation is probably due to the greater density of these masses.

(Received May 1, 1985; Accepted August 16, 1985)
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