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Plant and Cell Physiology, 1985, Vol. 26, No. 7 1233-1239
© 1985

Article

Identification of Gibberellins in Cotyledonary Embryos of Phaseolus coccineus L.

Piero Picciarelli and Amedeo Alpi

Cattedra di Fisiologia Vegetale, Facoltà di Agraria, Università degli Studi di Pisa Viale delle Piagge, 23, 56100 Pisa, Italy

Gibberellins present in cotyledonary embryos of Phaseolus coccineus L. have been identified by combined gas chromatography-mass spectrometry as GA1, GA4, GA5, GA6; also an unidentified gibberellin was present.

The total amount of gibberellins was estimated by gas chromatography to be 26.5 µg per g fresh weight; the individual gibberellins contributed to the total amount as follows: GA1 18.4µg, GA5 2.6 µg, GA4 2.3 µg, GA6 1.9 µg, unidentified GA 1.3 µg. Data are discussed in relation to previous results in P. coccineus seed as well as in relation to the embryo-suspensor system.

(Received December 12, 1985; Accepted July 8, 1985)
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