Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Wyndaele, R.
Right arrow Articles by de Greef, J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Wyndaele, R.
Right arrow Articles by de Greef, J.
Agricola
Right arrow Articles by Wyndaele, R.
Right arrow Articles by de Greef, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1985, Vol. 26, No. 6 1147-1154
© 1985

Article

Dynamics of Endogenous IAA and Cytokinins during the Growth Cycle of Soybean Crown Gall and Untransformed Callus

Rita Wyndaele1, Henri van Onckelen2,3, Jørgen Christiansen1, Patrick Rüdelsheim2,4, Robert Hermans2 and Jan de Greef2

1 Institute of Molecular Biology and Plant Physiology, Aarhus University DK-8000 Aarhus, Denmark
2 University of Antwerpen, UIA, Department of Biology B-2610 Wilrijk, Belgium

The dynamics of the endogenous IAA and cytokinin levels during the growth cycle of two soybean crown gall lines (green and pale) induced by a nop+ Agrobacterium tumefaciens C58 strain, were compared with an untransformed soybean callus line.

In both transformed tumor lines maximum cytokinin (essentially glucosyl-trans-zeatin) levels were attained in the beginning of the exponential growth phase, followed by a drastic decrease just before the stationary phase was reached. Quantitatively the green tumor line showed a 2–3 times higher cytokinin content compared with the pale line. In the untransformed soybean callus hardly any significant levels of cytokinin could be detected.

Analysis of endogenous IAA levels showed no difference between the two tumor lines and the untransformed callus tissue, all showing a low and constant level throughout the entire growth cycle.

The relevance of the endogenous accumulation of phytohormones in relation to the hormone autotrophic growth of transformed soybean tissue is discussed.

3 Senior Research Associate Nationaal Fonds Wetenschappelijk Onderzoek (N.F.W.O.).

4 Recipient of an Instituut voor Wetenschappelijk Onderzoek in Nijverheid en Landbouw (I.W.O.N.L.) grant.

(Received March 25, 1985; Accepted June 21, 1985)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.