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Plant and Cell Physiology, 1985, Vol. 26, No. 5 973-976
© 1985

Short Communication

Isolation and Culture of Protoplasts from Protonemata of Several Moss Species

Hideo Usui1 and Michio Ito2

1 Biological Institute, Faculty of Education, Mie University Kamihama, Tsu 514, Japan
2 Department of Biology, Faculty of Science, Nagoya University Chikusa, Nagoya 464, Japan

Protoplasts were isolated enzymatically from protonemata of several moss species. The protoplasts began to divide at limited frequencies within several days when cultured on White's inorganic medium supplemented with 0.3 M mannitol. Vigorous growth into filamentous protonemata was induced when they were transplanted onto media of lower osmolarity.

(Received January 28, 1985; Accepted May 13, 1985)
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