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Plant and Cell Physiology, 1985, Vol. 26, No. 5 881-891
© 1985

Article

Localization of Carbonic Anhydrase in Mesophyll Cells of Terrestrial C3 Plants in Relation to CO2 Assimilation

Mikio Tsuzuki1, Shigetoh Miyachi1 and Gerald E. Edwards2

1 Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
2 Botany Department and Institute of Biological Chemistry, Washington State University Pulluman, Washington 99164-4230, U.S.A.

The activity and intracellular compartmentation of carbonic anhydrase was examined in mesophyll protoplasts of several C3 terrestrial species including wheat, since this enzyme may facilitate diffusion of inorganic carbon in solution by converting CO2 to bicarbonate. Carbonic anhydrase was located in the mesophyll chloroplast with little or no activity in the cytosolic fraction. In wheat, carbonic anhydrase was absent in etiolated leaves and increased in the light during greening. Thus the enzyme may have a role in photosynthesis in the chloroplast but not in the cytosol of mesophyll cells of higher C3 plants.

The amount of CO2 required for half maximum rates of photosynthesis (under low O2) was about two-fold higher for isolated protoplasts than with isolated chloroplasts of wheat. The form of inorganic carbon taken up by protoplasts, like that of chloroplasts, is CO2. The results are discussed in relation to a possible resistance to CO2 transfer in the cytosol of mesophyll cells.

(Received February 25, 1985; Accepted May 7, 1985)
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