Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Drotar, A.
Right arrow Articles by Fall, R.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Drotar, A.
Right arrow Articles by Fall, R.
Agricola
Right arrow Articles by Drotar, A.
Right arrow Articles by Fall, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1985, Vol. 26, No. 5 847-854
© 1985

Article

Methylation of Xenobiotic Thiols by Euglena gracilis: Characterization of a Cytoplasmic Thiol Methyltransferase

AnnaMarie Drotar and Ray Fall

Department of Chemistry, and Cooperative Institute for Research in Environmental Sciences, University of Colorado Boulder, CO 80309 U.S.A.

The green alga Euglena gracilis contains a thiol methyltransferase that catalyzes the S-adenosylmethionine-dependent methylation of pentachlorobenzenethiol. The enzyme was localized in the cytoplasm and partially purified. The pH optimum for the enzyme was 6.5. The enzyme methylated a number of foreign thiols, but not the cellular thiols, glutathione or cysteine. Phenols and anilines were not substrates. When pentachloro-benzenethiol was the methyl acceptor the Km was found to be 82 µM and the corresponding Km for S-adenosylmethionine was 140 µM. The molecular weight of the enzyme was 21,000, as determined by gel filtration. A role for this enzyme in detoxifying xenobiotic thiols is proposed.

(Received September 28, 1984; Accepted April 25, 1985)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
M. L. Alvarez-Rodriguez, L. Lopez-Ocana, J. M. Lopez-Coronado, E. Rodriguez, M. J. Martinez, G. Larriba, and J.-J. R. Coque
Cork Taint of Wines: Role of the Filamentous Fungi Isolated from Cork in the Formation of 2,4,6-Trichloroanisole by O Methylation of 2,4,6-Trichlorophenol
Appl. Envir. Microbiol., December 1, 2002; 68(12): 5860 - 5869.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. James, K. D. Nolte, and A. D. Hanson
Purification and Properties of S-Adenosyl-L-methionine:L-Methionine S-Methyltransferase from Wollastonia biflora Leaves
J. Biol. Chem., September 22, 1995; 270(38): 22344 - 22350.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.